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Radioimmunoassay for human aldolase A
Clinica Chimica Acta; International Journal of Clinical Chemistry
|December 24, 1981
Summary
A new radioimmunoassay accurately measures aldolase A in human serum. This method is specific for aldolase A, enabling precise quantification in healthy individuals.
Area of Science:
- Biochemistry
- Immunology
- Clinical Chemistry
Background:
- Aldolase A is a key enzyme in glycolysis.
- Accurate quantification of aldolase A in serum is important for diagnostic purposes.
- Existing methods may lack specificity or sensitivity.
Purpose of the Study:
- To develop and validate a direct radioimmunoassay for quantifying aldolase A in human serum.
- To establish the specificity and sensitivity of the developed assay.
- To determine normal ranges of serum aldolase A in healthy individuals.
Main Methods:
- A double antibody radioimmunoassay was developed.
- Radioiodinated aldolase A4 homopolymer was used as the ligand.
- Chicken antibodies to aldolase A and rabbit antibodies to chicken IgG were employed.
Main Results:
- The assay demonstrated a lowest measurable amount of 2 ng (0.01 U).
- The radioimmunoassay showed high specificity for aldolase A, with no cross-reactivity to aldolase B or C.
- Serum aldolase A levels in 41 healthy subjects ranged from 130 to 210 ng/ml (mean 171 ± 39 ng/ml).
Conclusions:
- A specific and sensitive radioimmunoassay for human serum aldolase A has been successfully developed.
- This assay allows for reliable direct quantification of aldolase A in serum.
- The established normal ranges provide a reference for clinical applications.