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Related Experiment Videos

Soluble immune complexes binding to human monocytes and polymorphonuclear leucocytes

F G Cosio, S K Ackerman, S D Douglas

    Immunology
    |December 1, 1981
    PubMed
    Summary
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    Human immune complexes (IC) bind to monocytes and neutrophils. Factors like low pH and trypsin affect this binding, influencing immune cell interactions and potential therapeutic targets.

    Area of Science:

    • Immunology
    • Cell Biology

    Background:

    • Soluble human serum albumin anti-albumin immune complexes (IC) are involved in immune responses.
    • Monocytes and polymorphonuclear leucocytes (PMN) are key immune cells that interact with ICs.

    Purpose of the Study:

    • To investigate the binding characteristics of anti-albumin ICs to human monocytes and PMN.
    • To explore factors influencing IC binding, including pH and enzymatic treatment.

    Main Methods:

    • In vitro binding assays using freshly isolated human peripheral blood monocytes and PMN.
    • Saturation binding studies and inhibition assays with aggregated IgG subclasses.
    • Incubation at low pH (6.0) and treatment with trypsin.

    Main Results:

    Related Experiment Videos

  • Both monocytes and PMN exhibit saturable binding of ICs, with PMN having more binding sites.
  • Low pH (6.0) enhances IC binding through increased affinity in PMN and increased site number in monocytes.
  • Trypsin treatment differentially affects binding: suspension treatment increases sites and affinity, while adherent treatment primarily increases monocyte affinity.

    • Conclusions:

    • Human monocytes and PMN possess specific binding sites for anti-albumin ICs.
    • Environmental factors like pH and cell surface modifications (trypsin) modulate IC binding affinity and site availability, impacting immune cell function.