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Ascorbate uptake by isolated rat lung cells

J R Wright, V Castranova, H D Colby

    Journal of Applied Physiology: Respiratory, Environmental and Exercise Physiology
    |December 1, 1981
    PubMed
    Summary
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    Isolated rat lung cells concentrate ascorbate 16 times higher than plasma. This uptake is an energy-dependent, sodium-coupled carrier-mediated process, crucial for lung cell function.

    Area of Science:

    • Biochemistry
    • Cell Biology
    • Pulmonary Medicine

    Background:

    • Ascorbate (vitamin C) is vital for cellular functions.
    • Understanding ascorbate transport in lung cells is crucial for respiratory health.

    Purpose of the Study:

    • To quantify intracellular ascorbate levels in rat lung cells.
    • To investigate the mechanism of ascorbate influx into these cells.

    Main Methods:

    • Measurement of intracellular and plasma ascorbate concentrations.
    • Incubation of isolated lung cells with varying ascorbate levels.
    • Analysis of ascorbate uptake kinetics and inhibition studies.

    Main Results:

    • Intracellular ascorbate concentration (2.25 mM) was ~16 times higher than plasma (0.14 mM).

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  • Ascorbate influx showed linear increase up to 60 min at physiological concentrations.
  • Uptake exhibited saturation kinetics (Km = 0.16 mM) and was carrier-mediated (>90%).
  • Influx was inhibited by metabolic inhibitors, ouabain, and sodium removal.

    • Conclusions:

    • Rat lung cells possess an energy-dependent, Na+-coupled carrier system for ascorbate uptake.
    • This mechanism facilitates high intracellular ascorbate accumulation, essential for lung cell function.