Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Cerebral protective effect and radical scavenging action

H Yasuda, O Shimada, A Nakajima

    Journal of Neurochemistry
    |October 1, 1981
    PubMed
    Summary

    Nizofenone demonstrated anti-peroxidative activity in vitro, protecting mitochondria. However, this radical scavenging action showed no direct link to the cerebral protective effects of tested drugs.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Antiphospholipid syndrome with complete abdominal aorta occlusion and chondritis.

    Modern rheumatology·2014
    Same author

    Does "conversion chemotherapy" really improve survival in metastatic colorectal cancer patients with liver-limited disease?

    World journal of surgery·2013
    Same author

    Diagnosis of biliary cystadenoma by peroral video cholangioscopy.

    Endoscopy·2013
    Same author

    Vaccination against seasonal influenza is effective in Japanese patients with rheumatoid arthritis enrolled in a large observational cohort.

    Scandinavian journal of rheumatology·2013
    Same author

    Expression of the G72/G30 gene in transgenic mice induces behavioral changes.

    Molecular psychiatry·2013
    Same author

    Protection from non-alcoholic steatohepatitis and liver tumourigenesis in high fat-fed insulin receptor substrate-1-knockout mice despite insulin resistance.

    Diabetologia·2012

    Area of Science:

    • Biochemistry
    • Neuropharmacology
    • Mitochondrial research

    Background:

    • Oxidative stress contributes to cellular damage, particularly in the brain.
    • Investigating the mechanisms of cerebral protection is crucial for developing effective treatments.
    • Free radical scavenging is a proposed mechanism for neuroprotection.

    Purpose of the Study:

    • To investigate the role of radical scavenging in the cerebral protective effects of drugs.
    • To evaluate the anti-peroxidative activity of nizofenone.
    • To compare the radical scavenging capacity of nizofenone with known cerebral protective agents.

    Main Methods:

    • In vitro incubation of rat brain and liver mitochondria with ascorbic acid and Fe2+ to induce peroxidation.
    • Assessing mitochondrial disintegration via malondialdehyde formation and turbidity.
    • Testing the inhibitory effects of nizofenone and various barbiturates on mitochondrial peroxidation.
    • Evaluating free radical scavenging activity using diphenyl-p-picrylhydrazyl (DPPH).

    Main Results:

    • Ascorbic acid and Fe2+ induced peroxidative disintegration of rat brain mitochondria.
    • Nizofenone inhibited mitochondrial peroxidative disintegration in a dose-dependent manner (10-200 micro M).
    • Nizofenone's anti-peroxidative activity was comparable to alpha-tocopherol.
    • Thiopental showed slight efficiency, while other barbiturates and DMSO had no effect on mitochondrial peroxidation.
    • Nizofenone scavenged DPPH radicals, but barbiturates did not.

    Conclusions:

    • Nizofenone possesses significant anti-peroxidative properties.
    • The study suggests no direct correlation between free radical scavenging activity and the cerebral protective effects of the investigated drugs.
    • Further research is needed to elucidate the precise mechanisms of cerebral protection.

    Related Experiment Videos