Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Isolated heart myocytes: ultrastructural case study technique

T F Robinson, B S Hayward, J W Krueger

    Journal of Microscopy
    |November 1, 1981
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Coronary flow reserve of nonischemic heart failure.

    Congestive heart failure (Greenwich, Conn.)·2002
    Same author

    Persistent formation of angiotensin II despite treatment with maximally recommended doses of angiotensin converting enzyme inhibitors in patients with chronic heart failure.

    Journal of the renin-angiotensin-aldosterone system : JRAAS·2002
    Same author

    Cardiomyocyte aging is gender-dependent: the local IGF-1-IGF-1R system.

    Heart disease (Hagerstown, Md.)·2001
    Same author

    Perindopril treatment for congestive heart failure.

    The American journal of cardiology·2001
    Same author

    Ligand binding in the ferric and ferrous states of Paramecium hemoglobin.

    Biochemistry·2000
    Same author

    Angiotensin II receptor subtypes in the skeletal muscle vasculature of patients with severe congestive heart failure.

    Circulation·2000
    Same journal

    BioImageIT: A novel python-based architecture for reproducible bio-image workflows.

    Journal of microscopy·2026
    Same journal

    In operando imaging of the space-charge region in a 4H-SiC MOSCAP using STEM-EBIC.

    Journal of microscopy·2026
    Same journal

    The future of DXA: How AI is transforming bone health diagnostics.

    Journal of microscopy·2026
    Same journal

    The Origins of Ploem's Filter Cube: A Pandora's Box.

    Journal of microscopy·2026
    Same journal

    The reproducibility gap in graph neural network workflows for cell dynamics: A checklist-driven case study.

    Journal of microscopy·2026
    Same journal

    Assessing the reproducibility of a bioimage analysis workflow characterising tissue flow in Drosophila.

    Journal of microscopy·2026
    See all related articles

    Researchers developed a simple method to study rat heart muscle cells, correlating their electrical properties and structure. This technique minimizes cell shrinkage, offering a reliable way to analyze cardiomyocyte ultrastructure.

    Area of Science:

    • Cardiology
    • Cell Biology
    • Biophysics

    Background:

    • Studying isolated heart muscle cells (cardiomyocytes) is crucial for understanding cardiac function.
    • Previous electron microscopy methods for cardiomyocytes often involved heterogeneous cell populations or complex preparations.
    • A need exists for a simpler, cost-effective method to analyze cardiomyocyte structure and function.

    Purpose of the Study:

    • To develop and validate a straightforward, inexpensive method for the case study of enzymatically isolated rat ventricular cardiomyocytes.
    • To correlate live-state sarcomere length and electrical stimulatibility with ultrastructural features.
    • To assess cell shrinkage during sample preparation.

    Main Methods:

    • Enzymatic isolation of rat ventricular cardiomyocytes.

    Related Experiment Videos

  • Adaptation of cell monolayer preparation techniques for electron microscopy.
  • Controlled experimental conditions with varying calcium chloride (CaCl2) concentrations (0 mmol and 1 mmol).
  • Correlation of live-state measurements (sarcomere length, electrical stimulatibility) with ultrastructural analysis (myofilament disposition, cell coat integrity).
  • Direct determination of sample shrinkage using striated photographs.

    • Main Results:

    • A simple and inexpensive method for studying isolated rat cardiomyocytes was successfully developed.
    • The method allows for the correlation of live electrical properties with detailed ultrastructure.
    • Cell shrinkage during preparative steps was determined to be less than 5%.

    Conclusions:

    • The developed method offers a reliable and efficient approach for investigating cardiomyocyte structure-function relationships.
    • This technique minimizes artifacts like cell shrinkage, enhancing the accuracy of ultrastructural analysis.
    • The method is suitable for controlled studies investigating factors affecting cardiomyocyte contractility and ultrastructure.