Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Specimen heating during sputter-coating

A W Robards, A J Wilson, P Crosby

    Journal of Microscopy
    |November 1, 1981
    PubMed
    Summary
    This summary is machine-generated.

    Researchers developed a low-energy sputtering technique for thin gold films on frozen biological samples. This method minimizes heating, preserving specimen integrity for advanced microscopy and analysis.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Patient Experiences of Using Wearable Health Monitors During Cancer Treatment: A Qualitative Study.

    Clinical oncology (Royal College of Radiologists (Great Britain))·2024
    Same author

    Advanced Systemic Mastocytosis with associated haematological neoplasm: Treatment with avapritinib can facilitate successful bridge to allogeneic haematopoietic cell transplant.

    Current research in translational medicine·2023
    Same author

    Using genomic prediction to detect microevolutionary change of a quantitative trait.

    Proceedings. Biological sciences·2022
    Same author

    Excellent medium-term survival of an all-inside tensionable knotted suture device justifies repair of most meniscal tears encountered during reconstructive knee ligament surgery.

    Knee surgery, sports traumatology, arthroscopy : official journal of the ESSKA·2020
    Same author

    Social stress and glucocorticoids alter PERIOD2 rhythmicity in the liver, but not in the suprachiasmatic nucleus.

    Hormones and behavior·2020
    Same author

    First Report of Tomato spotted wilt virus in Common Agapanthus.

    Plant disease·2019
    Same journal

    In operando imaging of the space-charge region in a 4H-SiC MOSCAP using STEM-EBIC.

    Journal of microscopy·2026
    Same journal

    The future of DXA: How AI is transforming bone health diagnostics.

    Journal of microscopy·2026
    Same journal

    The Origins of Ploem's Filter Cube: A Pandora's Box.

    Journal of microscopy·2026
    Same journal

    The reproducibility gap in graph neural network workflows for cell dynamics: A checklist-driven case study.

    Journal of microscopy·2026
    Same journal

    Assessing the reproducibility of a bioimage analysis workflow characterising tissue flow in Drosophila.

    Journal of microscopy·2026
    Same journal

    Modular training resources for bioimage analysis.

    Journal of microscopy·2026
    See all related articles

    Area of Science:

    • Materials Science
    • Biophysics
    • Cryogenics

    Background:

    • Sputtering is crucial for preparing biological specimens for microscopy.
    • Conventional sputtering methods can cause significant heating, damaging delicate biological samples.
    • Low-temperature sputtering is needed to preserve sample integrity.

    Purpose of the Study:

    • To develop a low-energy sputtering technique for depositing gold films on frozen biological specimens.
    • To minimize heat generation during the sputtering process.
    • To achieve high-quality gold films suitable for cryo-electron microscopy and other low-temperature applications.

    Main Methods:

    • Utilized permanent magnets to confine plasma, reducing energy requirements for sputtering.
    • Employed a system operating at 250 V and 12-15 mA to achieve 15 nm gold films in 2-3 minutes.

    Related Experiment Videos

  • Conducted theoretical analysis of heat transfer under worst-case conditions.
  • Validated findings with practical experiments at both cryogenic (<120 K) and ambient temperatures.

    • Main Results:

    • Successfully deposited 15 nm gold films on frozen specimens within 2-3 minutes using low energy.
    • Theoretical calculations predicted a maximum specimen temperature increase of 6.0 K at equilibrium.
    • Practical results confirmed minimal temperature rise, supporting theoretical assumptions.
    • Demonstrated effective sputtering at ambient temperatures with negligible temperature increase (<3 K).

    Conclusions:

    • The developed low-energy sputtering technique effectively coats frozen biological specimens with gold films without causing thermal damage.
    • This method offers a reliable and efficient way to prepare samples for advanced imaging techniques.
    • The findings are applicable to both cryogenic and ambient temperature sputtering applications.