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A simple method for whole-cell preparation in electron microscopy

S Aizu, T Itoh, T Y Yamamoto

    Journal of Microscopy
    |November 1, 1981
    PubMed
    Summary

    This study presents a novel whole-cell preparation technique for electron microscopy, eliminating the need for gold or platinum grids. This simplified method cultures cells on formvar-coated coverslips for efficient sample processing and observation.

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    Area of Science:

    • Cell biology
    • Microscopy techniques

    Background:

    • Traditional whole-cell preparation for electron microscopy often requires specialized and costly materials like gold or platinum grids.
    • Developing simpler, more accessible methods for cell culture and preparation is crucial for broader research applications.

    Purpose of the Study:

    • To describe a straightforward method for whole-cell preparation for electron microscopy.
    • To eliminate the requirement of gold or platinum grids in the cell culture and preparation process.

    Main Methods:

    • Cells were cultured on formvar film supported by Thermonox coverslips with 3 mm pores.
    • The cultured cells underwent in situ fixation, staining, and dehydration.
    • Critical point drying was employed, followed by the application of mesh-cement to slot grids, which were then placed on the formvar film.

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    Main Results:

    • A simplified whole-cell preparation method was successfully developed.
    • The technique avoids the use of gold or platinum grids, utilizing formvar-coated coverslips instead.
    • Prepared samples on grids were ready for direct observation under the electron microscope.

    Conclusions:

    • This method offers a cost-effective and simpler alternative for whole-cell preparation in electron microscopy.
    • The technique facilitates efficient sample processing, making electron microscopy more accessible for cell biology research.