Related Experiment Video
Updated: Aug 15, 2026
22:27
Hi-C: A Method to Study the Three-dimensional Architecture of Genomes.
Published on: May 6, 2010
[Study of complexes of poly-L-histidine with DNA using hyperchromic spectra]
Molekuliarnaia Biologiia
|November 1, 1981
Abstract:
Complexes of DNA with poly-L-histidine in acidic media were investigated by the method of hyperchromic spectra. Making use of the least squares hyperchromic spectra were partitioned into the components corresponding to the melting of AT and GC base pairs and protonation of cytosine. The protonation of cytosine was found to be proportional to the fraction of broken GC base pairs and was not influenced by the presence of poly-L-histidine. Selectivity of poly-L-histidine towards the base pairs was found to be very weak or absent.
Related Concept Videos
01:01Euchromatin
The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions take up more dye, appearing darker, while the less-compact areas take up less dye and appear lighter. Based on the compaction level, chromatins are classified into two primary forms – euchromatin and heterochromatin.
Euchromatin is the less dense region of the chromatin and stains lighter. Euchromatin contains histone H3 extensively...
Euchromatin is the less dense region of the chromatin and stains lighter. Euchromatin contains histone H3 extensively...
02:38Heterochromatin
The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions that take up more dye are called heterochromatin. Heterochromatin is further classified into two forms – constitutive heterochromatin and facultative heterochromatin.
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at 9th...
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at 9th...