Met-enkephalin uptake by a synaptosome-enriched fraction of rat striatum

We describe the accumulation of tritiated Met-enkephalin by a synaptosome-enriched fraction of rat striatum. Specificity of Met-enkephalin uptake was established by thin layer chromatography. This uptake process is dependent on substrate concentration and is inhibited by excess unlabeled peptide. It requires energy; it is inhibited by coincubation with ouabain.