Related Experiment Video
Updated: Aug 10, 2026

Spectral Confocal Imaging of Fluorescently tagged Nicotinic Receptors in Knock-in Mice with Chronic Nicotine Administration
Published on: February 10, 2012
Central sites and mechanisms of action of nicotine
Abstract:
Research conducted in this laboratory over the last ten years has been directed towards determining possible CNS sites and mechanisms by which nicotine is producing its psychopharmacological effects. To accomplish these goals, a drug discrimination paradigm was utilized in which rats were trained to detect nicotine using a two-lever operant procedure. In this situation nicotine acted as a discriminative stimulus (DS) to correct lever responding. In other words rats had to be able to differentiate nicotine's effects from saline in order to obtain a food reinforcement. The ability of nicotine to exert its DS effects appear to be dependent upon a stimulation of central nicotinic-cholinergic receptors which are stereospecific to (-)-nicotine. Interestingly, the nicotine DS could not be mimicked or potentiated by elevating brain acetylcholine levels centrally suggesting that the receptor action was non-cholinergic. Additional studies indicated that nicotine is acting at both reticular formation and hippocampus sites. The hippocampal site of nicotine action also appears to be dependent on a dopamine neuron connection as well.
Related Concept Videos
Neurochemical Transmission: Sites of Drug Action
Cholinergic Receptors: Nicotinic
There are two types of nicotinic receptors: neuromuscular (NM/NM/N1) and neuronal (NN/NN/N2). The two families differ based on their location and selectivity to...
Direct-Acting Cholinergic Agonists: Chemistry and Structure-Activity Relationship
The direct-acting...
Drugs Acting on Autonomic Ganglia: Stimulants
Ganglionic stimulants activate NM nicotinic receptors in autonomic ganglia, falling into two categories: nicotine mimetics [e.g., lobeline, dimethylpiperazine, tetramethylammonium] and muscarinic receptor agonists [e.g., muscarine, methacholine]. The first category's action is rapid and blocked by nicotinic receptor antagonists, while the second category's action is delayed and blocked by atropine-like agents. Nicotine, an alkaloid, affects the heart rate by stimulating sympathetic or...
Adrenergic Agonists: Indirect-Acting Agents
One mechanism involves depleting stored catecholamines by displacing them from synaptic vesicles. These agents, known as "displacers," are transported into vesicles at the expense of noradrenaline. Examples include amphetamine and tyramine, which lack a catechol moiety, resulting in prolonged action, improved oral bioavailability, and...
CNS Depressants: Alcohol and Nicotine

