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Different antibody response in experimental and spontaneous glomerulonephritis
Renal Physiology
|January 1, 1980
Summary
Antibodies against glomerular basement membrane (GBM) are key in nephritis. In Goodpasture's syndrome, these antibodies target GBM glycoproteins, not collagen, causing kidney disease.
Area of Science:
- Nephrology
- Immunology
- Pathology
Background:
- Immune nephritis involves antibody responses to glomerular basement membrane (GBM) components.
- Previous studies show sheep immunized with GBM develop antibodies against collagen and glycoprotein portions.
Purpose of the Study:
- To monitor the antibody response against collagen and glycoprotein portions of GBM in nephritis.
- To compare antibody profiles in experimental sheep nephritis and human Goodpasture's syndrome.
Main Methods:
- Utilized type IV collagen from pepsin digests and collagenase digests of GBM for immunization and antibody detection.
- Employed immunofluorescence microscopy to demonstrate antibody adsorption in kidneys.
- Measured circulating antibodies and serum creatinine levels.
Main Results:
- Circulating antibodies against GBM structures in sheep were detected only after clinical disease onset.
- Antibodies eluted from sheep kidneys showed linear adsorption.
- In human Goodpasture's syndrome, circulating antibodies at diagnosis reacted exclusively with GBM glycoproteins, not type IV collagen.
Conclusions:
- The antibody response in nephritis differs between experimental models and human disease.
- Goodpasture's syndrome involves antibodies specifically targeting GBM glycoproteins.