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[Dynamic interaction between spermatozoa and the mucosa of the female genital tract in the mouse: study using
Abstract:
Submicroscopic alterations occurring in mouse spermatozoa during their stay in the female reproductive tract as revealed by scanning and transmission electron microscopy using ruthenium red as marker for glycosaminoglycans are described in the present investigation. The results show that the surface of the plasma membrane covering the acrosomal region of the spermatozoa found in the uterus at different intervals of time after copulation (between 10 minutes up to 20 hours), was provided with an increasing number of granules and small vesicles. Some of the surface structures appear gradually leading to perforation of discrete areas of plasma membranes in regions corresponding to the acrosomal region covering the head of spermatozoa. Also the use of ruthenium red shows a significant decrease of glycosaminoglycans and their sialic acid moieties that specifically precede further vesiculation and fenestration (perforation) of the plasma membrane covering the same acrosomal regions.
Insights
Mouse sperm undergo surface changes in the female reproductive tract. Ruthenium red staining reveals decreased glycosaminoglycans, preceding plasma membrane perforation in the acrosomal region.
Area of Science:
- Reproductive Biology
- Cell Biology
- Electron Microscopy
Context:
- Spermatozoa undergo significant modifications within the female reproductive tract post-insemination.
- Understanding these changes is crucial for reproductive success and fertility studies.
Purpose:
- To investigate submicroscopic alterations in mouse spermatozoa within the female reproductive tract.
- To utilize ruthenium red staining to visualize changes in glycosaminoglycans.
Summary:
- Scanning and transmission electron microscopy revealed progressive changes on the sperm plasma membrane surface in the uterus.
- An increase in granules and vesicles was observed, leading to plasma membrane perforation over the acrosomal region.
- Ruthenium red staining indicated a decrease in glycosaminoglycans and sialic acid, preceding membrane vesiculation and fenestration.
Impact:
- Provides detailed ultrastructural insights into sperm capacitation.
- Highlights the role of glycosaminoglycans in sperm membrane remodeling.
- Contributes to the understanding of male fertility mechanisms.