Comparison of direct and indirect ELISA for detecting antigenically related cucumoviruses

The suitability of two ELISA procedures for detecting serologically closely related as well as distantly related cucumoviruses has been compared. When antibodies to a single strain were used, closely related strains of cucumber mosaic virus could be detected by both direct and indirect ELISA. However, only the indirect ELISA procedure was capable of detecting distantly related viruses such as tomato aspermy, peanut stunt and cucumber mosaic viruses.