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Related Experiment Videos

Furosemide induced hepatotoxicity

R M Walker, T F McElligott

    The Journal of Pathology
    |December 1, 1981
    PubMed
    Summary
    This summary is machine-generated.

    Furosemide causes liver damage (hepatotoxicity) in mice, leading to cell death. The study reveals early changes in liver cells and suggests the plasma membrane plays a key role in this toxicity.

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    Area of Science:

    • Hepatotoxicity
    • Toxicology
    • Cell Biology

    Background:

    • Furosemide is a diuretic drug with known potential for adverse effects.
    • Understanding the mechanisms of drug-induced liver injury is crucial for patient safety.

    Purpose of the Study:

    • To investigate the sequential ultrastructural changes in the liver following furosemide administration.
    • To elucidate the role of the plasma membrane in furosemide-induced hepatotoxicity.

    Main Methods:

    • Swiss white mice were administered furosemide (400mg/kg ip).
    • Light and electron microscopy were used to examine liver tissue at various time points (1.5 and 3 hours post-administration).

    Main Results:

    • Early light microscopy findings included centrilobular glycogen depletion and cytoplasmic foaminess, progressing to congestion and hydropic necrosis.

    Related Experiment Videos

  • Electron microscopy revealed polyribosome disaggregation, endoplasmic reticulum vesiculation, and an endocytic origin for vacuolation.
  • Vacuolation correlated with microvilli loss, sinusoidal lining cell detachment, and erythrocyte entry into Disse space and vacuoles.

    • Conclusions:

    • Furosemide induces centrilobular necrosis in mice, characterized by specific ultrastructural changes.
    • The findings suggest that plasma membrane damage, including microvilli loss and vacuolation, is central to furosemide hepatotoxicity.
    • These mechanisms may be shared with other known membrane-targeting toxins.