Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Design of a single electrode voltage clamp

M Merickel

    Journal of Neuroscience Methods
    |February 1, 1980
    PubMed
    Summary

    This study introduces a novel single microelectrode voltage clamp for studying small cell membrane processes. This technique enables the investigation of electrically excitable cells previously inaccessible to traditional methods.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    What's in store for imaging workstations?

    Diagnostic imaging·1990
    Same author

    Evidence for an alteration of the tonotopic map in the gerbil cochlea during development.

    The Journal of comparative neurology·1989
    Same author

    Electrophysiology of human muscle in culture.

    Experimental neurology·1981
    Same author

    Cultured muscle from myotonic muscular dystrophy patients: altered membrane electrical properties.

    Proceedings of the National Academy of Sciences of the United States of America·1981
    Same author

    Investigation of burst generation by the electrically coupled cyberchron network in the snail Helisoma using a single-electrode voltage clamp.

    Journal of neurobiology·1980
    Same author

    Single cell isolation: a way to examine network interactions.

    Brain research·1979

    Area of Science:

    • Biophysics
    • Cellular Electrophysiology
    • Microelectrode Technology

    Background:

    • Traditional two-microelectrode voltage clamp systems cannot penetrate small cells.
    • Studying membrane processes in small cells is crucial for understanding cellular function.

    Purpose of the Study:

    • To present the design and construction of a single microelectrode voltage clamp.
    • To enable the study of membrane processes in small cells.

    Main Methods:

    • Utilizes a single microelectrode for alternating current injection and membrane potential sampling.
    • Employs electronic switching circuitry for time-sharing control.
    • Samples membrane potential after current pulse discharge from microelectrode capacitance.

    Main Results:

    • The single microelectrode voltage clamp can operate in current injection or voltage clamp modes.
    • In voltage clamp mode, steady-state membrane potential is reached within 2 msec for a 40 mV step.
    • The device is suitable for investigating slow current processes in small electrically excitable cells.

    Conclusions:

    • The single microelectrode voltage clamp is a significant advancement for studying small cell electrophysiology.
    • This technique expands the scope of voltage clamp technology to previously inaccessible cell types.
    • It holds potential for investigating slow current processes in various small excitable cells.

    Related Experiment Videos