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[Spontaneous L-transformation in actinomycetes]
Mikrobiologiia
|November 1, 1981
Summary
Spontaneous L-transformation, characterized by cell wall-deficient bacteria, was observed in Actinomadura and Nocardiopsis. These polymorphous cells exhibit unique internal structures and budding, typical of bacterial L-forms.
Area of Science:
- Microbiology
- Cell Biology
Background:
- Actinomadura and Nocardiopsis are genera of bacteria.
- Bacterial L-forms are cells that have lost their cell wall.
Purpose of the Study:
- To describe the phenomenon of spontaneous L-transformation in Actinomadura and Nocardiopsis.
- To characterize the morphological features of these transformed cells.
Main Methods:
- Culturing of Actinomadura and Nocardiopsis for two days.
- Microscopic examination of cell morphology.
Main Results:
- Observed polymorphous cells (0.5-1.5 µm) lacking a cell wall.
- Noted nucleoid occupying a large cell portion, with peripheral cytoplasm.
- Identified cytoplasmic membranous structures (five-layer membranes) and observed budding.
- Confirmed these features are typical of bacterial L-forms and absent in intact cell wall bacteria.
Conclusions:
- Spontaneous L-transformation occurs in Actinomadura and Nocardiopsis.
- The observed cells represent bacterial L-forms with distinct ultrastructural characteristics.
- These findings contribute to understanding bacterial cell wall variability and L-form biology.