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Epidermal feet in pupal segment morphogenesis

M Locke, P Huie

    Tissue & Cell
    |January 1, 1981
    PubMed
    Summary
    This summary is machine-generated.

    Insect epidermal cells possess unique cytoskeletal feet crucial for metamorphic shape changes. These structures, visualized using a novel lead salt technique, extend and contract in response to the hormone 20-hydroxyecdysone.

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    Area of Science:

    • Developmental Biology
    • Cell Biology
    • Insect Morphology

    Background:

    • Insect integumental epithelia feature epidermal cells with basal cytoskeletal extensions, termed feet.
    • These feet resemble cellular processes observed in tissue culture but are difficult to visualize within the intact epithelium.

    Purpose of the Study:

    • To develop a novel method for visualizing epidermal cell feet in insect integument.
    • To investigate the role of these feet in metamorphic changes of segment shape.
    • To explore the hormonal regulation of epidermal feet.

    Main Methods:

    • A new procedure involving glutaraldehyde fixation, tannic acid penetration, lead salt mordanting, and osmium binding was employed.
    • This technique renders individual cell outlines visible within the epithelium, mimicking tissue culture preparations.

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  • Observations were made on Calpodes larvae during different developmental stages.

    • Main Results:

    • The procedure successfully visualized epidermal cell feet, revealing their branched morphology and extent.
    • Epidermal feet were identified as metamorphic structures formed after pupal commitment.
    • Feet extend axially along the segment gradient at low 20-hydroxyecdysone titers and contract at high titers, correlating with segment shortening.

    Conclusions:

    • The developed method effectively demonstrates the presence and morphology of epidermal cell feet.
    • These feet are essential for metamorphic alterations in insect segment shape.
    • The extension and contraction of epidermal feet are regulated by 20-hydroxyecdysone levels, highlighting their dynamic role in insect metamorphosis.