Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Ethanol 'dose-dependent' elimination: Michaelis-Menten v classical kinetic analysis

R E Rangno, J H Kreeft, D S Sitar

    British Journal of Clinical Pharmacology
    |November 1, 1981
    PubMed
    Summary

    This study compared linear regression and Michaelis-Menten models for ethanol elimination. Both methods accurately determined maximal elimination rates, but linear regression overestimated ethanol

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Use of antiviral prophylaxis in influenza outbreaks in long term care facilities.

    The Canadian journal of infectious diseases = Journal canadien des maladies infectieuses·2007
    Same author

    British and Canadian views on the ethics of paediatric clinical trials.

    European journal of clinical pharmacology·2007
    Same author

    How long after drug ingestion is activated charcoal still effective?

    Journal of toxicology. Clinical toxicology·2002
    Same author

    Comparative bioavailability of acyclovir from oral valacyclovir and acyclovir in patients treated for recurrent genital herpes simplex virus infection.

    The Canadian journal of clinical pharmacology = Journal canadien de pharmacologie clinique·2001
    Same author

    Interaction between N-acetylcysteine and activated charcoal: implications for the treatment of acetaminophen poisoning.

    Pharmacotherapy·2001
    Same author

    Population pharmacokinetics of fentanyl in healthy volunteers.

    Journal of clinical pharmacology·2001

    Area of Science:

    • Pharmacokinetics
    • Biomedical Engineering
    • Drug Metabolism

    Background:

    • Ethanol elimination kinetics are crucial for understanding alcohol's effects.
    • Accurate modeling of ethanol pharmacokinetics aids in clinical and forensic toxicology.
    • Classical and mechanistic models offer different approaches to analyzing drug concentration-time data.

    Purpose of the Study:

    • To compare the efficacy of classical linear regression and a Michaelis-Menten elimination model for analyzing ethanol pharmacokinetics.
    • To evaluate how these models handle varying ethanol doses and post-prandial effects.
    • To determine if one model provides a more accurate estimation of ethanol distribution and elimination parameters.

    Main Methods:

    • Eight healthy volunteers received intravenous ethanol doses (0.375–0.75 g/kg).

    Related Experiment Videos

  • Four subjects received oral ethanol doses (0.5–1.25 g/kg).
  • Time-plasma concentration profiles were analyzed using a two-compartment Michaelis-Menten model and classical linear regression.

    • Main Results:

    • The Michaelis-Menten model yielded specific parameters (median absorption constant 1.29 h⁻¹, Vmax 0.12 g h⁻¹ kg⁻¹, Km 0.03 g/L).
    • Classical techniques provided a slope of 0.20 g L⁻¹ h⁻¹ and overestimated the volume of distribution by 17%.
    • Both techniques equally determined maximal ethanol elimination rates, but neither explained post-prandial slope changes.

    Conclusions:

    • Maximal ethanol elimination rates are reliably determined by both classical and Michaelis-Menten models.
    • Classical linear regression overestimates the volume of distribution for ethanol.
    • Neither model adequately explains transient decreases in elimination slope or dose-dependent increases at high concentrations.