The nucleotide sequence of 5S ribosomal RNA from slime mold Physarum polycephalum

The nucleotide sequence of 5S ribosomal RNA from plasmodia of the slime mold Physarum polycephalum was determined as pppGGAUGCGGC CAUACUAAGG 20 AGAAAGCACC 30 UCAUCCCGUC 40 CGAUCUGAGA 50 AGUUAAGCUC 60 CUUCAGGCGU 70 GGUUAGUACU 80 GGGGUGGGGG 90 ACCACCUGGG 100 AAUCCCACGU 110 GCUGCAUUCU 120 Uoh by chemical and enzymatic gel sequencing technics using 3' and 5' end-labeled RNA. This RNA is very different from 5S rRNA of the cellular slime mold Dictyostelium discoideum (36 nucleotides are different), and shows greater similarity to 5S rRNAs from Protozoa and Metazoa than to those from fungi.