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Monocyte function in normal adults. Simplified methods of separation and testing

J W Lawton, I D Gardner

    Scandinavian Journal of Haematology
    |August 1, 1981
    PubMed
    Summary
    This summary is machine-generated.

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    This study presents a simple, high-yield method for isolating human blood monocytes. The procedure enables reliable functional testing, including adhesion, chemotaxis, and phagocytosis, with established normal values for healthy adults.

    Area of Science:

    • Immunology
    • Cell Biology
    • Hematology

    Background:

    • Monocytes are crucial immune cells involved in various physiological and pathological processes.
    • Efficient isolation of pure monocytes is essential for accurate functional studies.
    • Existing monocyte isolation methods can be time-consuming or yield suboptimal results.

    Purpose of the Study:

    • To develop and validate a streamlined, one-step method for isolating human blood monocytes.
    • To assess the yield and purity of monocytes obtained using the modified Ficoll-Hypaque centrifugation technique.
    • To establish a panel of reliable and reproducible functional assays for isolated monocytes.

    Main Methods:

    • Human peripheral blood monocytes were isolated using a one-step centrifugation over modified Ficoll-Hypaque.

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  • Monocyte yield and proportion were calculated based on blood leucocyte and differential counts.
  • Functional assays included adhesion to glass, spreading on glass, chemotaxis under agarose, and phagocytosis/killing of Candida albicans.

    • Main Results:

    • The modified Ficoll-Hypaque method achieved high monocyte proportions (mean 54%) and yields (0.22-0.98 x 10(9)/L).
    • Yields were approximately 2.5 times the theoretical maximum, indicating efficient isolation.
    • Functional tests demonstrated high reproducibility and comparable results to previously reported methods.
    • Normal values for monocyte function were established from 35 healthy Chinese adults.

    Conclusions:

    • The described one-step centrifugation method provides a highly efficient and reproducible means of isolating human blood monocytes.
    • The validated functional assays are suitable for routine use in research and diagnostics.
    • This method facilitates further investigation into monocyte biology and function in various health conditions.