Semen collection and evaluation in the ram. The preparation of spermatozoa for morphological examination

Tail and mid-piece morphology of ram spermatozoa were compared using wet preparations of semen diluted in buffered formal saline at temperatures of 10 degrees C and 65 degrees C. The temperature of the diluent did not affect the occurrence of abnormalities. Tail and mid-piece morphology were also examined in semen smears stained by a modified Williams' stain using glass slides at temperatures of 10 degrees C, 38 degrees C and 65 degrees C. The occurrence of the abnormalities was not affected by the slide temperature. The occurrence of tail and mid-piece abnormalities was compared using the 2 methods of preparation. Only the occurrence of distal cytoplasmic droplets was affected by the method of preparation. In the stained smear preparations, most of the distal cytoplasmic droplets were lost. However, only a few of the proximal droplets were lost when this method was used.