Hemoglobin Shuangfeng (alpha 27 (B8) Glu substituting for Lys): a new unstable hemoglobin variant

An unstable hemoglobin variant was found in a 24-year-old Chinese of Han origin. It had an electrophoretic mobility slightly faster than that of Hb A2, but could not be separated from it completely by cellulose acetate electrophoresis (pH 8.6). Chromatography on DE-52 column showed that the variant amounted to approximately 13% of the total hemoglobin. Structural analysis showed that the hemoglobin had the structure alpha 27 (B8) Glu substituting for Lys. It was named Hemoglobin Shuangfeng. The alpha 27 Glu residue is an invariable residue located near the alpha 1 beta 1 contact.