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Updated: Aug 9, 2026

Quantifying Microglia Morphology from Photomicrographs of Immunohistochemistry Prepared Tissue Using ImageJ
Published on: June 5, 2018
Image enhancement for light microscopy
Abstract:
Digitized light microscope images of low contrast histological samples were processed through image enhancement computer techniques. Original digital data and their square root transformations were linearly expanded to produce a wider range of brightness levels in the enhanced images. These treatments resulted in significantly improved images compared to those obtained by direct photomicrography.
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