Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Structural comparisons of superoxide dismutases

J I Harris, A D Auffret, F D Northrop

    European Journal of Biochemistry
    |May 1, 1980
    PubMed
    Summary

    Amino-terminal sequences of superoxide dismutase enzymes from seven microbes reveal evolutionary relationships. Iron and manganese types show sequence overlap, indicating broad variation across bacterial species.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Workplace social support in job satisfaction among veterans with posttraumatic stress symptoms: A preliminary correlational study.

    PloS one·2017
    Same author

    Comparison of a Nerve Gas Detoxifying Enzyme from Squid and from Pseudomonas diminuta.

    The Biological bulletin·2017
    Same author

    A PHYSICO-CHEMICAL STUDY OF THE MECHANICAL PROPERTIES OF LOW AND INTERMEDIATE MOISTURE FOODS.

    Journal of texture studies·2017
    Same author

    A Commensal Strain of Staphylococcus epidermidis Overexpresses Membrane Proteins Associated with Pathogenesis When Grown in Biofilms.

    The Journal of membrane biology·2015
    Same author

    Nucleotide sequences of the genes for the alpha, beta and epsilon subunits of wheat chloroplast ATP synthase.

    Plant molecular biology·2013
    Same author

    Mitochondrial nucleoid interacting proteins support mitochondrial protein synthesis.

    Nucleic acids research·2012

    Area of Science:

    • Biochemistry
    • Microbiology
    • Enzymology

    Background:

    • Superoxide dismutases (SODs) are critical enzymes protecting cells from oxidative stress.
    • Two major families of SODs exist: copper/zinc (Cu/Zn) and manganese/iron (Mn/Fe).
    • Understanding the evolutionary diversity of SODs is crucial for comprehending their protective functions across different life forms.

    Purpose of the Study:

    • To determine the amino-terminal sequences of superoxide dismutase enzymes from seven diverse microorganisms.
    • To compare these sequences with known SOD families, particularly Mn/Fe types.
    • To investigate the evolutionary relationships and sequence variations among bacterial SODs, including those from anaerobic phototrophs.

    Main Methods:

    • Amino-terminal sequencing of purified superoxide dismutase enzymes.
    • Bioinformatic analysis and comparison of determined sequences with existing databases.
    • Secondary structure predictions to identify conserved structural motifs.

    Main Results:

    • The amino-terminal sequences of seven microbial superoxide dismutases were determined, including novel sequences from anaerobic phototrophs.
    • Five enzymes contained iron, and two contained manganese; all were related but distinct from the Cu/Zn SOD family.
    • No clear sequence distinction was found between iron and manganese types, and significant variation existed among bacterial enzymes.
    • Enzymes from anaerobes did not form a distinct, closely related group compared to aerobes.
    • Two conserved histidine residues, potentially metal ligands, were identified in the same alpha-helix across all analyzed proteins.

    Conclusions:

    • The study reveals extensive sequence diversity within the Mn/Fe superoxide dismutase family across bacteria.
    • Conserved histidine residues suggest a common metal-binding mechanism in these enzymes.
    • Evolutionary relationships indicate a complex diversification of superoxide dismutases, not strictly divided by metal cofactor or oxygen requirement.

    Related Experiment Videos