Synthesis and properties of human growth hormone fragments

The solid phase synthesis of fragments 75-120-NH2 and 73-128-GlyNH2 of the human growth hormone sequence is described. Purification was by solvent extraction, gel filtration and partition chromatography. No growth-promoting activity was detected in any of the peptides by the rat body-growth assay. An immunochemically reactive area was located in the sequence 73-128 when tested by complement fixation assays.