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Leucocyte migration agarose test to study cell-mediated immunity in amoebiasis
The Southeast Asian Journal of Tropical Medicine and Public Health
|September 1, 1981
Summary
The leucocyte migration agarose test (LMAT) effectively measures cell-mediated immunity (CMI) in amoebic liver abscess patients. LMAT positivity increased with antigen concentration and peaked 31-60 days post-illness onset.
Area of Science:
- Immunology
- Infectious Diseases
- Parasitology
Background:
- Amoebic liver abscess (ALA) is a significant global health concern.
- Accurate assessment of cell-mediated immunity (CMI) is crucial for understanding ALA pathogenesis.
- Existing diagnostic methods may not fully capture the immune response dynamics in ALA.
Purpose of the Study:
- To quantitatively evaluate the magnitude of CMI in patients with ALA using the leucocyte migration agarose test (LMAT).
- To compare LMAT results with the indirect haemagglutination (IHA) test in ALA patients.
- To establish the time course of CMI response in ALA.
Main Methods:
- The leucocyte migration agarose test (LMAT) was employed to assess CMI.
- 35 patients diagnosed with amoebic liver abscess were included.
- 22 healthy individuals served as controls.
Main Results:
- LMAT demonstrated higher positivity rates with increased amoeba extract concentration (65.7% at 200 µg, 85.7% at 400 µg).
- All healthy controls tested negative with LMAT.
- CMI response, as measured by LMAT, peaked between 31-60 days after illness onset.
Conclusions:
- LMAT is a valuable tool for quantifying CMI in amoebic liver abscess.
- The CMI response in ALA develops over time, with a significant response observed after 30 days.
- LMAT provides a distinct measure of immune response compared to serological tests like IHA.