A study on the relative turnover of muscle proteins. Comparison of turnover rates in a single step procedure

In order to enable a direct comparison of the turnover rates of all muscle proteins we introduce a single step procedure. This procedure is based on gel filtration in the presence of a detergent SDS (sodium dodecyl sulphate) which solubilises all muscle proteins. Although the separation is still very crude, some preliminary conclusions may be drawn abut the relative turnover rates of myofibrillar proteins. In our opinion it is unlikely that the myofibril turns over as a unit.