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Related Experiment Videos

Bacteriocin and hemolysin from Streptococcus faecium

S Nakagawa, Y Matsuo

    Antimicrobial Agents and Chemotherapy
    |October 1, 1981
    PubMed
    Summary

    Streptococcus faecium produces bacteriocins and hemolysins, which were separated by gel filtration. Hemolytic activity was observed in non-bacteriocinogenic mutants, indicating distinct production pathways.

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    Area of Science:

    • Microbiology
    • Biochemistry

    Background:

    • Streptococcus faecium is a bacterium known to produce antimicrobial substances.
    • Bacteriocins and hemolysins are toxins with distinct biological activities.

    Purpose of the Study:

    • To partially separate bacteriocin and hemolysin from Streptococcus faecium.
    • To characterize the molecular weights and production requirements of these substances.

    Main Methods:

    • Gel filtration using Ultrogel AcA-22 for partial separation.
    • Analysis of hemolytic activity in non-bacteriocinogenic mutants.

    Main Results:

    • Bacteriocin molecular weight was approximately 300,000 Da; hemolysin molecular weight was 220,000 Da.
    • Hemolytic activity was present in mutants lacking bacteriocin production.
    • Hemolysin production was glucose-dependent, while bacteriocin production was not.

    Conclusions:

    • Bacteriocin and hemolysin from Streptococcus faecium are distinct molecules with different molecular weights.
    • Hemolytic activity is independent of bacteriocin production in this strain.
    • Glucose plays a differential role in the biosynthesis of hemolysin and bacteriocin.

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