An experimental model to study bile and exocrine pancreatic secretion from mice

An in vivo experimental model to obtain exocrine pancreatic secretion and bile from mice has been developed. It consists of a microsurgical procedure in anesthetized mice. The bile-pancreatic common duct and the duodenum were individualized, isolated, and cannulated using a stereoscopic microscope to obtain pure pancreatic juice, pure bile, bile-pancreatic juice, and duodenal contents. Intravenous injection of secretin as a pancreatic secretion stimulant was used with this experimental model. Fluid color an enzymatic activity were used as indicators of the fluid purity. In order to evaluate the overall procedure, mortality under surgery and volumes of samples obtained (expressed as microliters/30 minutes) before and after administration of secretin was measured. Results from a total of 524 mice of the BALB/c and DBA/2J-cri inbred strains were evaluated.