A simple procedure to obtain continuous cell lines from bovine peripheral blood leucocytes

A method is described by which cell lines can be readily developed from bovine peripheral leucocytes. Fifteen cell lines have been developed from 25 attempts, passage levels up to 60 being reached. The cell lines are aneuploid and predominantly epithelial, show split ratio capabilities of 1:4 to give monolayers with 5 days of routine passage, and have high resistance to laboratory contamination with bacterial and fungal agents. Data are given concerning establishment, morphology, viral susceptibility and chromosomal counts of established cell lines.