[Humoral regulation of granulopoiesis in experimental inflammation]

The model of experimentally induced inflammation demand for granulocytes was used to study the changes in granulocytic cell compartments and the level of humoral regulators of granulopoiesis. The experimental inflammation in rats induced by i.p. injection of 3.5% solution of polivinylpyrrolidone (PVP) provoked during 24 hours intensive mobilisation of granulocytes into peritoneal exudate and the intensive proliferation of granulocytic cells in the bone marrow between 18-24 hours. When the bone marrow of CBA mice was cultivated in diffusion chambers implanted into rats with experimentally induced inflammation, stimulation of granulopoiesis was noted during the period of five days. The results indirectly indicate that the level of granulopoiesis stimulating factor was increased in the diffusion chambers host animals. It was demonstrated also that rat's serum obtained 20 hours after injection of PVP (leukophoretic serum) stimulates the proliferation of granulocytes in the bone marrow of normal CBA mice cultivated in diffusion chambers. The level of stimulation was confirmed to be dose dependent. Presented data indicate that in the conditions of increased demand for granulocytes, the level of humoral regulator--stimulator of proliferation of granulocytic cells--is increased in the circulation.