[Experience in the preparation of leukocytes and thrombocytes for transfusion]

The authors convey their experience gained during the work with Haemonetics M-30 Separator. They had performed 80 separations in total. They had separated leukocytes and thrombocytes simultaneously for 67 times. Most of the donors (51) had been stimulated by corticosteroids in order to obtain the increase of yield. The average concentrate quantity was 355 +/- 91 ml. The leukocytes yield in the concentrate was means = 27,3 +/- 7,8 x 10(9)/1, with 19,2 +/- 5,6 x 10(9)/1 (70%) of polymorphonuclears. The thrombocytes yield was means = 7,80 +/- +/- 1,83 x 10(11)/1. Only thrombocytes were separated 13 times. The average concentrate quantity was 273 +/- 77 ml. The thrombocytes yield in the concentrate was 8,94 +/- 1,26 x 10(11)/1. During the study, the symptoms and signs of hypotony appeared in one the donors.