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Related Experiment Videos

Collagen-platelet interaction. Type V(A-B) collagen induced platelet aggregation

T M Chiang, C L Mainardi, J M Seyer

    The Journal of Laboratory and Clinical Medicine
    |January 1, 1980
    PubMed
    Summary

    Type V collagen, in its fibrillar form, triggers human platelet aggregation and serotonin release. Its amorphous or soluble forms do not affect platelets, highlighting the importance of its quaternary structure.

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    Area of Science:

    • Biochemistry
    • Cell Biology
    • Hematology

    Background:

    • Type V collagen is a recently identified collagen.
    • Its role in platelet function is not fully understood.
    • Previous studies suggested potential platelet interactions.

    Purpose of the Study:

    • To investigate the effect of type V collagen on human platelet aggregation.
    • To determine if the native fibrillar structure of type V collagen is necessary for platelet interaction.
    • To differentiate the effects of type V collagen from potential contaminants.

    Main Methods:

    • Type V collagen was prepared and its solubility properties exploited to distinguish it from interstitial collagens.
    • Type V collagen was subjected to enzymatic digestion with synovial collagenase.

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  • Platelet aggregation and serotonin release assays were performed.
  • Purity of type V collagen was confirmed using SDS-PAGE of CNBr digests.

    • Main Results:

    • Native, fibrillar type V collagen mediated human platelet aggregation and serotonin release.
    • Soluble or amorphous forms of type V collagen showed no detectable effect on platelets.
    • Solubility differences confirmed type V collagen's distinctness from interstitial collagens.
    • Enzymatic digestion of contaminants did not abolish platelet-aggregating properties.

    Conclusions:

    • The quaternary structure of type V collagen is critical for its recognition by platelet membranes.
    • Fibrillar assembly is essential for type V collagen's pro-aggregatory effect on human platelets.
    • Type V collagen represents a novel mediator of platelet activation.