Related Experiment Video

Updated: Aug 3, 2026

Determining the Reactivity and Titre of Serum using a Haemagglutination Assay
05:59

Determining the Reactivity and Titre of Serum using a Haemagglutination Assay

Published on: January 29, 2010

A clinical evaluation of a card agglutination test for rheumatoid factor

S L Hansen, F D Mazer, H F Klinefelter

    American Journal of Clinical Pathology
    |January 1, 1980
    PubMed

    Insights

    The Macro-Vue Card Test for Rheumatoid Factor offers reproducible results and detects more positive sera compared to other commercial kits. This rheumatoid factor test provides reliable qualitative and quantitative analysis for patient diagnosis.

    Area of Science:

    • Immunology
    • Clinical Chemistry
    • Diagnostic Assays

    Background:

    • Rheumatoid factor (RF) is a key biomarker in diagnosing rheumatoid arthritis.
    • Accurate and reproducible RF detection is crucial for effective patient management.
    • Evaluating new diagnostic tools is essential for improving laboratory diagnostics.

    Purpose of the Study:

    • To evaluate the performance of the new Hynson, Westcott, and Dunning Macro-Vue Card Test for Rheumatoid Factor.
    • To compare the Macro-Vue test with other commercially available rheumatoid factor assays.
    • To assess the qualitative and quantitative capabilities of the Macro-Vue RF Card Test.

    Main Methods:

    • A comparative study was conducted using coded sera from 195 patients.
    • Four commercial rheumatoid factor kits were evaluated: Hyland RA Test (latex), Behring-Rapi/tex RF Test (latex), Wampole Rheumaton (sheep erythrocytes), and HWD-Macro-Vue RF Card Test (charcoal).
    • Qualitative and quantitative analyses were performed according to manufacturers' instructions, with reproducibility assessed using known positive sera.

    Main Results:

    • The Macro-Vue RF Card Test demonstrated comparable performance to latex-based systems (Behring and Hyland).
    • The Macro-Vue test identified a higher percentage of positive sera than the Wampole test.
    • Titers determined by the Macro-Vue test showed superior day-to-day reproducibility compared to all other evaluated kits.

    Conclusions:

    • The Macro-Vue Card Test for Rheumatoid Factor is a reliable diagnostic tool for both qualitative and quantitative RF detection.
    • Its high reproducibility makes it a valuable option for clinical laboratories seeking consistent rheumatoid factor testing.
    • The Macro-Vue test offers a competitive alternative to existing commercial rheumatoid factor assays, particularly in terms of titer stability.

    More Related Videos

    Methods for Quantitative Detection of Antibody-induced Complement Activation on Red Blood Cells06:29

    Methods for Quantitative Detection of Antibody-induced Complement Activation on Red Blood Cells

    Published on: January 29, 2014

    An Immunohistopathologic Study to Profile the Folate Receptor Beta Macrophage and Vascular Immune Microenvironment in Giant Cell Arteritis06:35

    An Immunohistopathologic Study to Profile the Folate Receptor Beta Macrophage and Vascular Immune Microenvironment in Giant Cell Arteritis

    Published on: February 8, 2019

    Related Concept Videos

    Aortic Regurgitation II: Clinical Features and Diagnostic Tests01:22

    Aortic Regurgitation II: Clinical Features and Diagnostic Tests

    Aortic valve regurgitation (AR) occurs when the aortic valve fails to close properly, allowing blood to flow backward from the aorta into the left ventricle. This backflow can result in two distinct clinical presentations: acute and chronic AR, each characterized by its own set of symptoms and physical findings.Acute Aortic RegurgitationAcute AR presents with a sudden onset of severe symptoms. Patients typically experience profound dyspnea (shortness of breath), chest pain, and signs of left...
    Myocarditis II: Clinical Features and Diagnostic Tests01:27

    Myocarditis II: Clinical Features and Diagnostic Tests

    Myocarditis is an inflammation of the heart muscle. The symptoms vary widely, encompassing asymptomatic presentations to severe, acute manifestations.Clinical PresentationAsymptomatic cases: In some instances, myocarditis may be asymptomatic, with the infection resolving without intervention. These cases often go undetected unless discovered incidentally through diagnostic imaging or tests conducted for other reasons.General Early Symptoms: Early symptoms of myocarditis are non-specific and can...
    Rheumatic Heart Disease I: Introduction01:23

    Rheumatic Heart Disease I: Introduction

    Rheumatic heart disease or RHD is a chronic condition that results from rheumatic fever, causing permanent damage to the heart valves.Etiology and Risk FactorsIt primarily arises from rheumatic fever, an inflammatory disease that can develop after untreated or inadequately treated group A streptococcal (GAS) pharyngitis. Streptococcus spreads through direct contact with oral or respiratory secretions. While the bacteria are the causative agents, factors like malnutrition, overcrowding, poor...
    Dysrhythmias V: Evaluating Dysrhythmias01:30

    Dysrhythmias V: Evaluating Dysrhythmias

    Dysrhythmias, also known as arrhythmias, are disturbances in the heart's rhythm that range from benign to life-threatening. A thorough evaluation is crucial for appropriate management and involves a comprehensive medical history, physical examination, and various diagnostic tests.Medical HistorySymptoms: Collect detailed information on palpitations, dizziness, syncope, chest pain, and fatigue. Note their onset, frequency, and triggers.Previous Cardiac Issues: Document any history of heart...