Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Liposome filtration. Dependence on transition temperature

A M Brendzel, I F Miller

    Biochimica Et Biophysica Acta
    |February 15, 1980
    PubMed
    Summary

    Liposome filtration behavior changes significantly with temperature relative to their gel-liquid crystal transition. Above this transition, liposomes become unfilterable, suggesting a new method for controlling liposome size.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Adaptability of acrylic resin for ponties and copings.

    Dental survey·2010
    Same author

    Applied psychology in the dental treatment room.

    Oral hygiene·2010
    Same author

    Particle image velocimetry investigation of intravalvular flow fields of a bileaflet mechanical heart valve in a pulsatile flow.

    The Journal of heart valve disease·2000
    Same author

    The new St. Jude Medical regent mechanical heart valve: laboratory measurements of hydrodynamic performance.

    The Journal of heart valve disease·2000
    Same author

    Controlled dissolution from wax-coated aerosol particles in canine lungs.

    Journal of applied physiology (Bethesda, Md. : 1985)·1998
    Same author

    Effect of particle morphology on emitted dose of fatty acid-treated disodium cromoglycate powder aerosols.

    Pharmaceutical development and technology·1997

    Area of Science:

    • Physical chemistry
    • Materials science
    • Biophysics

    Background:

    • Liposomes are versatile lipid-based nanoparticles used in drug delivery and research.
    • Understanding liposome behavior during processing, such as filtration, is crucial for consistent formulation.
    • The gel-liquid crystal transition temperature significantly impacts liposome properties and membrane fluidity.

    Purpose of the Study:

    • To investigate the effect of filtration temperature relative to the liposome gel-liquid crystal transition on liposome filtration behavior.
    • To determine if liposome size distributions can be accurately measured by filtration across the transition temperature.
    • To explore the potential of this filtration behavior as a method for liposome size control.

    Main Methods:

    • Liposomes were prepared using vortexing.
    • Filtration experiments were conducted using polycarbonate surface retention membranes.
    • Filtration behavior was analyzed at temperatures below and above the liposome gel-liquid crystal transition temperature.
    • Liposome size distributions were determined using cumulative volume distributions.

    Main Results:

    • Liposomes exhibited distinct filtration behaviors depending on the filtration temperature relative to the gel-liquid crystal transition.
    • Below the transition temperature, liposomes were filterable, and their size distributions were log-normal.
    • Above the transition temperature, liposomes were unfilterable, with smaller liposomes forming until a limiting size was reached.
    • This indicates that filtration is unsuitable for determining liposome size distributions in the liquid crystalline state.

    Conclusions:

    • Liposome filtration behavior is intrinsically linked to the gel-liquid crystal transition temperature.
    • Filtration cannot reliably determine liposome size distributions in the liquid crystalline phase.
    • This temperature-dependent filtration property offers a novel approach for controlling liposome size and has broad implications for lipid membrane science.

    Related Experiment Videos