Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Sub-thylakoid fractions containing ribosomes

M M Margulies, J S Weistrop

    Biochimica Et Biophysica Acta
    |January 1, 1980
    PubMed
    Summary

    Researchers isolated thylakoid-bound ribosomes using detergents, creating

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Sequence similarity between Photosystems I and II. Identification of a Photosystem I reaction center transmembrane helix that is similar to transmembrane helix IV of the D2 subunit of Photosystem II and the M subunit of the non-sulfur purple and flexible green bacteria.

    Photosynthesis research·2014
    Same author

    Photosystem I reaction center polypeptides of spinach are synthesized on thylakoid-bound ribosomes.

    Archives of biochemistry and biophysics·1987
    Same author

    Identification of polypeptides of photosystem I reaction center as the products of chloroplast genes PS1A1 and PS1A2.

    Biochemical and biophysical research communications·1987
    Same author

    Synthesis of large subunit of ribulosebisphosphate carboxylase by thylakoid-bound polyribosomes from spinach chloroplasts.

    Archives of biochemistry and biophysics·1986
    Same author

    Importance of sodium dodecyl sulfate source to electrophoretic separations of thylakoid polypeptides.

    Analytical biochemistry·1984
    Same author

    Synthesis of photosynthetic membrane proteins directed by RNA from rough thylakoids of Chlamydomonas reinhardtii.

    European journal of biochemistry·1983

    Area of Science:

    • * Molecular Biology
    • * Biochemistry
    • * Plant Cell Biology

    Background:

    • * Thylakoid membranes in chloroplasts host bound ribosomes involved in protein synthesis.
    • * Understanding the composition and attachment of these ribosomes is crucial for chloroplast research.

    Purpose of the Study:

    • * To characterize a sub-membrane fraction containing thylakoid-bound ribosomes, termed 'pseudopolysomes'.
    • * To investigate the composition and properties of these pseudopolysome fractions.

    Main Methods:

    • * Treatment of thylakoids with detergents (Nonidet P-40, Triton X-100) to isolate pseudopolysomes.
    • * Analysis of pseudopolysome composition using buoyant density centrifugation and protein characterization.
    • * Differential release of ribosomes and subunits using specific agents (trypsin, KCl, puromycin).

    Main Results:

    • * Detergent treatment yielded pseudopolysome fractions rich in thylakoid-bound ribosomes but low in chlorophyll.
    • * Pseudopolysomes contained specific thylakoid polypeptides but lacked a major 50,000 Mr polypeptide.
    • * Ribosomes could be released from pseudopolysomes and thylakoids by specific treatments, indicating bound complexes.

    Conclusions:

    • * Pseudopolysomes represent a fraction of thylakoid membranes with attached ribosomes.
    • * This fraction provides insights into the sites of ribosome binding on thylakoid membranes.
    • * The findings contribute to understanding chloroplast protein synthesis and membrane-ribosome interactions.

    Related Experiment Videos