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Related Experiment Videos

Improved protein-binding radioassay for plasma cortisol

H Novacenko, F Halpern, E J Sachar

    Clinical Chemistry
    |February 1, 1980
    PubMed
    Summary

    This study presents a faster method for measuring plasma cortisol using filter paper and dichloromethane extraction. The improved radioassay allows for rapid, accurate analysis of numerous samples for clinical research.

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    Area of Science:

    • Clinical Chemistry
    • Endocrinology
    • Analytical Chemistry

    Background:

    • Accurate measurement of plasma cortisol is crucial for diagnosing adrenal disorders.
    • Existing methods for cortisol analysis can be time-consuming and labor-intensive.
    • There is a need for rapid, high-throughput assays for total plasma cortisol.

    Purpose of the Study:

    • To describe an improved competitive protein-binding radioassay for total plasma cortisol.
    • To enable rapid analysis of a large number of plasma samples.
    • To validate the accuracy and reliability of the new method.

    Main Methods:

    • Utilized a Whatman 3MM filter-paper strip for direct plasma application (0.1 mL).
    • Employed a single dichloromethane extraction of the filter paper.
    • Assayed the extracted residue directly using a micro-method based on Murphy's technique.

    Main Results:

    • Achieved excellent agreement with the original Murphy method (ethanol extraction), with a correlation coefficient (r) of 0.988.
    • Demonstrated the capability to easily assay one hundred samples in duplicate simultaneously.
    • The modified method allows for significantly faster sample processing.

    Conclusions:

    • The improved radioassay provides a rapid and efficient method for total plasma cortisol determination.
    • This technique is suitable for high-volume sample analysis in clinical and research settings.
    • The method maintains high accuracy comparable to established protocols.

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