Related Experiment Video
Updated: Sep 5, 2026

Murine Kidney Transplant Technique
Published on: October 20, 2015
[Design and construction of a kidney preservation unit (Laboratory Models LM 1 and LM 2) and its components]
Abstract:
By combination of the adequate construction elements (cryostate, warmth exchanger, roll pump) the laboratory model of unit for the preservation of kidneys for animal experimental investigations was established. For this purpose a special organ container made of piacryl was constructed in two models, which with double walls does not need a particular isolation and, therefore, is freely accessible for manipulations during the experiment. Measurings of the horizontal and vertical distribution of the temperature in the organ container resulted in a decrease of temperature of 5--6 degrees (single bottom of the container) or 1--2 degrees (double chilled bottom of the container). The report of the courses of temperature during an organ perfusion resulted in a constancy of the average temperature of 6--8 degrees. The kidney preservation unit has proved in a long-term experiment during 24 hours.
Related Concept Videos
Kidney Transplant I: Introduction
Kidney Transplant II: Surgical Procedure

