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Related Experiment Videos

Serum gentamicin assay by a radiometric procedure

B A Gunn, S L Brown, C S Otey

    American Journal of Clinical Pathology
    |February 1, 1980
    PubMed
    Summary

    A new BACTEC radiometric assay for gentamicin serum levels is fast and reproducible. However, it shows significant differences at concentrations above 4 microgram/ml compared to other methods.

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    Area of Science:

    • Clinical Chemistry
    • Microbiology
    • Pharmacokinetics

    Background:

    • Accurate determination of gentamicin serum levels is crucial for therapeutic drug monitoring.
    • Existing microbiologic assays and radioimmunoassay (RIA) methods have limitations in speed or cost.
    • Radiometric methods offer potential for rapid and sensitive drug level determination.

    Purpose of the Study:

    • To evaluate a new radiometric (BACTEC) assay for gentamicin serum level determination.
    • To compare the BACTEC assay's performance against established microbiologic assays and RIA.
    • To assess the accuracy, speed, and reproducibility of the BACTEC method.

    Main Methods:

    • A 14C-urea substrate and Proteus species culture were used in the BACTEC assay.
    • Gentamicin levels in serum were determined using the BACTEC assay, three non-radiometric microbiologic assays, and RIA.

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  • Assay performance was compared across different gentamicin concentration ranges.

    • Main Results:

    • The BACTEC assay was found to be simple, fast, and reproducible.
    • At gentamicin concentrations < 4 microgram/ml, BACTEC accuracy was comparable to other assays.
    • At gentamicin concentrations > 4 microgram/ml, BACTEC showed significant differences, reading lower than RIA and other assays.

    Conclusions:

    • The BACTEC assay is a viable alternative to RIA for laboratories with radiometric equipment, especially for batch processing.
    • Due to cost, BACTEC is less practical than rapid, inexpensive microbiologic assays for routine gentamicin monitoring.
    • The BACTEC assay's accuracy is concentration-dependent, requiring careful consideration for therapeutic drug monitoring.