Replication of eubenangee virus in Culicoides nuberculosus (Mg.) and Culicoides variipennis (Coq.)

Eubenangee virus multiplied to a high titre in both Culicoides variipennis and C. nuberculosus after intrathoracic inoculation but only in C. varriipennis after oral ingestion. 7.9 per cent of the orally infected C. variipennis supported virus replication and transmission of virus was demonstrated after 15 days' incubation at 26 degrees C. The significance of low infection rates when assessing vector potential in the laboratory and the relevance of these infection rates to the field situation are also discussed.