Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Evidence for active phospholipase A in mouse plasma

M P Gillett, E M Costa, J S Owen

    Biochimica Et Biophysica Acta
    |February 22, 1980
    PubMed
    Summary

    Mouse plasma contains active phospholipase A enzymes, unlike rat and human plasma. This enzyme facilitates phosphatidylcholine breakdown, impacting overall phospholipid levels in mice.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Survival and flight ability of Apis mellifera after exposure to anthranilic diamide insecticides.

    Brazilian journal of biology = Revista brasleira de biologia·2025
    Same author

    Organic fertilization in soybean cultivation and forage grown in succession.

    Brazilian journal of biology = Revista brasleira de biologia·2025
    Same author

    Adherence to inhaled corticosteroids and long-acting β2-agonists in asthma: A MASK-air study.

    Pulmonology·2023
    Same author

    Socioeconomic inequalities and temporomandibular joint disorders in adolescents: contributions from a Maranhão cohort.

    Community dental health·2021
    Same author

    A Brazilian regional basic diet-induced chronic malnutrition drives liver inflammation with higher ApoA-I activity in C57BL6J mice.

    Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas·2020
    Same author

    Impact of endostatin gene therapy on myeloid-derived suppressor cells from a metastatic renal cell carcinoma.

    Experimental oncology·2018

    Area of Science:

    • Biochemistry
    • Enzymology
    • Comparative Physiology

    Background:

    • Lecithin:cholesterol acyltransferase (LCAT) plays a crucial role in lipoprotein metabolism.
    • Phospholipid remodeling in plasma is influenced by enzymatic activity.
    • Species-specific differences in plasma enzyme activity can impact lipid profiles.

    Purpose of the Study:

    • To investigate the activity of phospholipase A and lysophosphatidylcholine hydrolase in mouse, rat, and human plasma.
    • To determine the role of these enzymes in phospholipid metabolism.
    • To compare enzymatic activity across different mammalian species.

    Main Methods:

    • Plasma samples from mouse, rat, and human were treated to inhibit LCAT activity.
    • Incubation at 37°C to observe changes in phospholipid concentrations.
    • Use of [U-14C]phosphatidylcholine to trace metabolic pathways in disulphide-treated mouse plasma.

    Main Results:

    • Disulphide-treated mouse plasma showed significant utilization of phosphatidylcholine and formation of lysophosphatidylcholine.
    • A decrease in total phospholipid concentration was observed in mouse plasma.
    • Radioactivity was recovered in lysophosphatidylcholine, non-esterified fatty acid, and glycero-3-phosphocholine fractions in mouse plasma.
    • Phospholipase A activity was detected in mouse plasma but not in rat or human plasma.

    Conclusions:

    • Phospholipase A and lysophosphatidylcholine hydrolase enzymes are active in mouse plasma.
    • Phospholipase A is either absent or inactive in rat and human plasma.
    • These findings highlight species-specific differences in plasma enzymatic activity involved in phospholipid metabolism.

    Related Experiment Videos