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Solid-phase synthesis of porcine vasoactive intestinal peptide
International Journal of Peptide and Protein Research
|January 1, 1980
Summary
Researchers synthesized porcine vasoactive peptide (VIP) and confirmed its purity and biological activity. The study also identified a potential deamidated contaminant forming during storage.
Area of Science:
- Biochemistry
- Peptide Synthesis
- Pharmacology
Background:
- Vasoactive intestinal peptide (VIP) is a peptide hormone with diverse physiological roles.
- Understanding the synthesis and stability of VIP is crucial for its therapeutic applications.
Purpose of the Study:
- To synthesize porcine VIP and characterize its purity and biological activity.
- To investigate the stability of synthesized VIP under storage conditions.
Main Methods:
- Solid-phase peptide synthesis using a benzhydrylamine resin support.
- Purification via ion-exchange and partition chromatography.
- Homogeneity assessment using High-Performance Liquid Chromatography (HPLC) and cyanogen bromide cleavage fragment analysis.
Main Results:
- Successfully synthesized and purified porcine VIP with a 16% yield.
- The purified VIP demonstrated characteristic inhibition of pentagastrin-induced gastric acid release in dogs.
- HPLC analysis revealed the slow formation of a deamidated contaminant in stored lyophilized VIP.
Conclusions:
- The synthesis and purification of porcine VIP were successful, yielding a biologically active peptide.
- Storage of lyophilized porcine VIP can lead to the formation of a deamidated contaminant, impacting long-term stability.
