Light scattering studies on alpha-crystallin from bovine eye lens

Using the usual angular light scattering technique, we have measured the concentration dependence of reduced scattering intensity of fractionated bovine lens alpha-crystallin (alpha A-chain) in the presence of 30 mM calcium chloride and 0.1 M sodium chloride. The inverse intensity of scattered light at zero angle per unit concentration of alpha-crystallin has a significant upward curvature with increasing alpha-crystallin concentration. The third virial coefficient determined from this upward curvature was 1.24 x 10(-3) (mol 1/2 ml 1/2 g-1).