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Updated: Aug 3, 2026

Plunge Freezing: A Tool for the Ultrastructural and Immunolocalization Studies of Suspension Cells in Transmission Electron Microscopy
Published on: May 5, 2017
Polymeric cryoprotectants in the preservation of biological ultrastructure. III. Morphological aspects
Abstract:
Two high molecular weight polymers, polyvinylpyrrolidone (PVP) and hydroxyethyl starch (HES), have been used as cryoprotectants for preparing specimens to be freeze fractured. Solutions of 25% (w/w) suppress the formation of intracellular ice in single cells and tissue blocks from both plants and animals to the extent that fine structural details of the cell can be elucidates. The mode of action of these cryoprotectants, together with the structures they reveal and the peculiar advantages attached to their use, is discussed.
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