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UV-light-induced mutations in synchronous CHO cells

H J Burki, C K Lam, R D Wood

    Mutation Research
    |February 1, 1980
    PubMed
    Summary
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    Germicidal UV light exposure affects Chinese hamster ovary (CHO) cell survival and mutation induction. Cell cycle stage significantly influences sensitivity to UV-induced reproductive death and mutations, with distinct responses for different mutagenic markers.

    Area of Science:

    • Cell Biology
    • Radiation Biology
    • Molecular Toxicology

    Background:

    • Understanding cellular responses to DNA damage is crucial for assessing risks associated with radiation exposure.
    • Chinese hamster ovary (CHO) cells are a widely used model system for studying DNA repair and mutagenesis.

    Purpose of the Study:

    • To investigate the fluence response curves for cell killing and the induction of resistance to specific mutagens in asynchronous and synchronous CHO cells following UV irradiation.
    • To determine the cell cycle dependence of UV-induced mutations and reproductive cell death.

    Main Methods:

    • Irradiation of asynchronous and synchronous CHO cells with germicidal UV light.
    • Determination of cell killing and induction of resistance to 6-thioguanine, ouabain, and diphtheria toxin.

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  • Analysis of cell cycle-specific responses for mutation induction and reproductive death.

    • Main Results:

    • UV irradiation showed a sigmoidal response for cell killing in asynchronous populations (D0 = 6 J/m2).
    • Mutation induction by UV light was linear for 6-thioguanine, ouabain, and diphtheria toxin resistance up to 17 J/m2.
    • Both reproductive death and mutation induction were cell cycle-dependent, with late G1 and early S phases being most sensitive.

    Conclusions:

    • Cell cycle stage significantly impacts CHO cell sensitivity to UV-induced reproductive death and mutation induction.
    • Different mutagenic endpoints exhibit distinct cell cycle-dependent responses to UV radiation.
    • Comparison with other mutagens suggests agent-specific and endpoint-specific age responses for mutation induction.