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Related Experiment Videos

The nucleolus during epidermal development in an insect

M Locke, P Huie

    Tissue & Cell
    |January 1, 1980
    PubMed
    Summary

    Calpodes epidermal cells exhibit distinct RNA synthesis phases during molting, regulated by ecdysteroid hormones. These phases involve significant nucleolar changes and the transport of molecular precursors via nuclear envelope microvesicles.

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    Area of Science:

    • Cell Biology
    • Developmental Biology
    • Insect Physiology

    Background:

    • Calpodes epidermis undergoes cyclical development during intermoult and moult phases.
    • These phases are characterized by specific patterns of RNA synthesis and nucleolar morphology.

    Purpose of the Study:

    • To investigate the cellular mechanisms underlying epidermal cell development in Calpodes.
    • To elucidate the role of RNA synthesis, nucleolar activity, and ecdysteroid hormones in molting.

    Main Methods:

    • Observation of epidermal cell development in the fifth stadium of Calpodes.
    • Analysis of RNA synthesis, nucleolar structure, and nuclear envelope morphology.
    • Correlation of cellular events with haemolymph ecdysteroid titres.

    Main Results:

    • Two distinct phases of epidermal cell development were identified, marked by elevated RNA synthesis and nucleolar elaboration.
    • Nucleolar morphology changed dynamically, with apparent nucleoli increasing from two to eight and back.
    • Ecdysteroid titres preceded nucleolar changes, and specific granules (ribosomal precursors and m-RNA precursors) were observed within the nucleolus and nuclear envelope.
    • Microvesicles within the nuclear envelope suggest a mechanism for molecular transport, potentially involving ecdysteroids.

    Conclusions:

    • The study reveals a coordinated cellular response to ecdysteroids during molting in Calpodes.
    • Nucleolar activity and the transport of molecular precursors via the nuclear envelope are critical for epidermal cell development and molting.

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