Utilization of a glycol-stabilized liquid NAD for the measurement of three enzymes on the GEMSAEC

Some analytical parameters have been investigated for a recently described stabilized liquid coenzyme technology in which water-free NAD is dissolved in 1,2 propanediol. Correlation for 108 specimens assayed for AST, ALT and LD with a reference method in which glycol-based NAD was absent was greater than or equal to 0.998 with near identical reproducibility over a period of at least 107 days. Mean recovery of exogenous serum enzymes in this linear kinetic assay is 103%. With the option of mixing only the volume of reagent needed for the enzymatic assay, waste can be eliminated as compared to more costly preparations stabilized by lyophilization. Hazards from an impure water supply are avoided since no reconstituting volume is required.