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Related Experiment Videos

Indium (111In)-labelled human platelets: optimal method

R J Hawker, L M Hawker, A R Wilkinson

    Clinical Science (London, England : 1979)
    |March 1, 1980
    PubMed
    Summary
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    This study presents a simple 45-minute laboratory method for labeling human platelets with Indium-111 oxine, suitable for routine clinical use. Labeled platelets maintain normal function and exhibit an average in vivo survival time of 8.44 days in healthy volunteers.

    Area of Science:

    • Nuclear Medicine
    • Hematology
    • Biotechnology

    Background:

    • Accurate assessment of platelet kinetics is crucial for diagnosing and managing various hematological disorders.
    • Existing methods for platelet labeling may be time-consuming or require large blood volumes, limiting their routine clinical applicability.

    Purpose of the Study:

    • To develop and validate a rapid, efficient, and clinically applicable laboratory method for labeling human platelets with Indium-111 oxine.
    • To evaluate the functional integrity and in vivo survival of platelets labeled using the described method.

    Main Methods:

    • A 45-minute laboratory procedure for labeling human platelets with Indium-111 oxine using a small blood volume (26 ml).
    • Assessment of platelet aggregation responses to adenosine diphosphate and collagen post-labeling.

    Related Experiment Videos

  • Quantification of Indium-111 oxine release during platelet secretory function.
  • Determination of platelet survival time in vivo in healthy volunteers using radiolabeled platelets.

    • Main Results:

    • High labeling efficiencies (90.1 +/- 4.29%) were achieved within 60 seconds using normal concentrations of plasma-free platelet suspensions.
    • Labeled platelets demonstrated aggregation responses comparable to normal platelet-rich plasma.
    • Minimal release (<5%) of Indium-111 oxine was observed during platelet secretory function.
    • In vivo platelet survival in healthy volunteers followed a linear function with a mean survival time of 8.44 +/- 0.18 days.

    Conclusions:

    • The described 45-minute Indium-111 oxine labeling method is simple, efficient, and suitable for routine clinical use.
    • Platelets labeled with Indium-111 oxine retain their functional integrity and exhibit a normal survival profile in vivo.
    • This method provides a reliable tool for studying platelet kinetics and evaluating platelet function in clinical settings.