Efficiency of medium utilization by human diploid fibroblasts in culture

Growth of two human diploid cell strains were studied in relation to medium formulation, supplementation and inoculum size. MRC-5 cells were found to give greatly improved yields in Dulbecco Modified Eagle's medium, while a foreskin strain, HFS-30, showed only a slight effect. Supplementation of nutrient by the addition of fresh medium after two days incubation and the use of small inocula, gave enhanced yields and medium utilisation in both cell types.