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Isolation and partial characterization of two plasmid deoxyribonucleic acids from endosymbiotic bacteria of Amoeba
Journal of Bacteriology
|March 1, 1980
Abstract:
Obligatory endosymbiotic bacteria in a strain of Amoeba proteus were found to harbor two distinct species of plasmid, pHJ11 and pHJ12. Their molecular weights were 39 x 10(6) and 14 x 10(6), respectively.
Insights
Obligatory endosymbiotic bacteria within Amoeba proteus host harbor two distinct plasmid species. These plasmids, pHJ11 and pHJ12, were characterized by their molecular weights.
Area of Science:
- Microbiology
- Molecular Biology
- Cell Biology
Background:
- Amoeba proteus are single-celled eukaryotes.
- Obligatory endosymbiotic bacteria reside within certain strains of A. proteus.
- Plasmids are extrachromosomal DNA molecules found in bacteria.
Purpose of the Study:
- To identify and characterize plasmids harbored by endosymbiotic bacteria in Amoeba proteus.
- To determine the molecular weights of these plasmids.
Main Methods:
- Isolation of endosymbiotic bacteria from Amoeba proteus.
- Plasmid DNA extraction and purification.
- Molecular weight determination of plasmids (e.g., using gel electrophoresis or other established methods).
Main Results:
- Two distinct plasmid species were identified in the endosymbiotic bacteria.
- Plasmid pHJ11 has a molecular weight of 39 x 10^6 Daltons.
- Plasmid pHJ12 has a molecular weight of 14 x 10^6 Daltons.
Conclusions:
- Endosymbiotic bacteria in Amoeba proteus possess unique plasmid profiles.
- The characterized plasmids, pHJ11 and pHJ12, represent significant genetic elements within these symbionts.